Thermo Fisher Scientific SCNN1B Monoclonal Antibody (7B8), PerCP
SCNN1B 단백질을 인식하는 PerCP 결합 단일클론 항체로, Western blot 및 IHC에 최적화되어 있습니다. 마우스 반응성, IgG1 아이소타입, Protein G 정제, 4°C 보관 조건. 나트륨 채널 연구 및 신장, 폐, 대장 조직 분석에 적합.
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Thermo Fisher Scientific SCNN1B Monoclonal Antibody (7B8), PerCP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:150 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7B8 |
| Immunogen | Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617–638) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931808 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-45354 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 87 kDa.
Target Information
Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls sodium reabsorption in kidney, colon, lung, and sweat glands, and plays a role in taste perception.
Forms a heterotetramer of two alpha, one beta, and one gamma subunit; a delta subunit can replace the alpha subunit. Interacts with WW domains of NEDD4, NEDD4L, WWP1, and WWP2.
Defects in SCNN1B cause autosomal recessive pseudohypoaldosteronism type 1 (PHA1) and Liddle syndrome, both involving abnormal sodium channel regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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