Thermo Fisher Scientific SCNN1B Monoclonal Antibody (16E4), FITC
SCNN1B 단백질을 인식하는 FITC 결합 단일클론 항체. Western blot에서 1:1,000 희석으로 사용 가능. Protein G 정제 및 무보존제 액상 형태. 4°C 암소 보관, 연구용 전용. 다양한 형광 표지 형식 제공.
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Applications and Tested Dilution
- Application: Western Blot (WB)
- Tested Dilution: 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16E4 |
| Immunogen | Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617–638) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS (pH 7.4) with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931810 |
Available Formats
- APC (Cat. MA5-45355)
- PerCP (Cat. MA5-45357)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45356 was sufficient for detection of ENaC beta in 15 µg of mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 87 kDa.
Target Information
Sodium-permeable, non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of luminal sodium and water through the apical membrane of epithelial cells, controlling sodium reabsorption in kidney, colon, lung, and sweat glands. Plays a role in taste perception.
The channel is a heterotetramer of two alpha, one beta, and one gamma subunit (a delta subunit can replace alpha). Interacts with WW domains of NEDD4, NEDD4L, WWP1, and WWP2.
Associated Diseases:
- Autosomal recessive pseudohypoaldosteronism type 1 (PHA1)
- Liddle syndrome (autosomal dominant pseudoaldosteronism with hypertension and hypokalemic alkalosis)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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