Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Biotin, eBioscience

Thermo Fisher Scientific CD95 (APO-1/Fas) Monoclonal Antibody (15A7), Biotin, eBioscience

Applications

• Reported Application: Flow Cytometry
• Tested Dilution: ≤ 0.5 µg/test
• Publications: View 15 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• PE
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation

Product Specific Information

The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40–50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes, and fibroblasts. Interaction of CD95 with its ligand (FasL) induces apoptosis and plays a role in peripheral tolerance.
15A7 reacts strongly with mouse CD95 in the BALB/c strain and more weakly in C57Bl/6. It does not induce apoptosis of Fas-expressing cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on BALB/c splenocytes and thymocytes; use ≤ 0.5 µg/test (100 µL final volume). Optimal titration recommended.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein functioning as a cell death receptor in the TNFR superfamily. Soluble FAS (~26 kDa) also exists. Stimulation leads to aggregation of FADD domains, formation of DISC, and activation of caspases, triggering apoptosis.
FAS can activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and transduce proliferative signals in fibroblasts and T cells. Multiple alternatively spliced isoforms exist; soluble forms may inhibit apoptosis mediated by full-length FAS.
The Fas/FasL system is implicated in diseases such as AIDS, hepatitis, and cancer, and is involved in antiviral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.