Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), Biotin, eBioscience
Rat CD106(VCAM-1)을 인식하는 Biotin 결합 단클론 항체로, 염증 반응 및 세포 부착 연구에 적합. Flow cytometry, IHC, ICC 등 다양한 응용 가능. 0.5 mg/mL 농도의 액상 제품으로 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD106 (VCAM-1) Monoclonal Antibody (eBioMR106 (MR106)), Biotin, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (PFA fixed) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry | 0.25 µg/test | View 2 publications |
| Functional Assay | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMR106 (MR106) |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_529469 |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The eBioMR106 monoclonal antibody reacts with rat CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1), a 110 kDa transmembrane glycoprotein expressed by myeloid lineage and bone marrow stromal cells. Endothelial cells constitutively express low levels of CD106 and further upregulate VCAM-1 upon cytokine stimulation. CD106 binds to integrin α4β1 (VLA-4, CD49d/CD29) and integrin α4β7 (LPAM-1), which are important in early lymphocyte and myeloid development. Cytokine-mediated upregulation of CD106 on endothelial cells suggests a role in inflammatory response.
Applications Reported: This antibody has been reported for use in flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of rat bone marrow cells at ≤0.25 µg per test (100 µL final volume). Cell number should be empirically determined (10⁵–10⁸ cells/test). Antibody should be titrated for optimal performance.
Target Information
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. It regulates leukocyte migration, leukocyte-endothelial adhesion, and signal transduction, and may play roles in inflammatory diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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