Thermo Fisher Scientific CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), Biotin, eBioscience
인간 CD104 (Integrin beta 4)을 인식하는 단클론 항체로, Biotin으로 표지된 제품입니다. Flow cytometry, IHC, ICC 등 다양한 응용에 적합하며, 0.25 µg/test로 사용 가능합니다. 암세포 및 상피세포 연구에 활용되며, Affinity chromatography로 정제된 고순도 항체입니다.
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Thermo Fisher Scientific CD104 (Integrin beta 4) Monoclonal Antibody (439-9B), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent | - |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 0.25 µg/test | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG2b |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 439-9B |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1210583 |
| Filtration | 0.2 µm post-manufacturing filtered |
Available Formats
Product Specific Information
The monoclonal antibody 439-9B recognizes human CD104 (Integrin beta 4), a 202 kDa subunit that associates with integrin alpha 6 (CD49f) to form the laminin receptor.
Expression is found on epithelial and tumor cells, and is involved in cell-matrix and cell-cell interactions.
CD104 plays a significant role in tumorigenicity and crossreacts to bovine.
Applications Reported:
- Flow cytometric analysis
- Immunohistology staining (frozen and paraffin tissue sections)
Applications Tested:
Tested by flow cytometric analysis of EDTA-treated A549 cell line.
Recommended usage ≤ 0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Careful titration recommended for optimal performance.
Target Information
Integrins are heterodimeric transmembrane glycoprotein receptors composed of alpha and beta subunits.
Integrin beta 4 acts as a laminin receptor, associating with alpha 6 subunit, and plays a pivotal role in carcinoma biology.
Mutations in this gene are linked to epidermolysis bullosa with pyloric atresia.
Multiple alternatively spliced transcript variants encode distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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