Takara His60 Ni Superflow Cartridges using FPLC

His 60 Ni Superflow, the highest-capacity resin to date that provides great purity, is now available in prefilled 1-ml and 5-ml cartridges for his-tagged protein purification on any LC system, such as ÄKTA or FPLC, or for manual purification using a syringe. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

His 60 Ni Superflow, the highest-capacity resin to date that provides great purity, is now available in prefilled 1-ml and 5-ml cartridges for his-tagged protein purification on any LC system, such as ÄKTA or FPLC, or for manual purification using a syringe. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses.

Protein purification principle

His60 Ni Protein Purification Resin, which is based on a modified Ni-IDA resin, provides remarkable selectivity and reliability when is it used to purify proteins containing an affinity tag of six or more consecutive histidine residues—such as the 6xHis tag or other his-tags. This technology allows one-step purification of almost any his-tagged protein from any expression system, under native or denaturing conditions. The His60 Ni Superflow purification resin has low nickel ion leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins).

His-tagged protein purification procedure

The purification of recombinant his-tagged proteins consists of four steps: cell lysis/extraction, binding, washing, and elution. His60 Nickel allows one-step protein purification under either native or denaturing conditions. The resin is compatible with multiple denaturants and detergents. Please see user manual for complete reagent compatibility list. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH. To protect your his-tag protein from degradation it is recommended to use protease inhibitors (ProteoGuard) during the purification process.

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Overview

• Up to 60 mg of recombinant his-tagged protein (1-ml cartridge)
• Up to 300 mg of recombinant his-tagged protein (5-ml cartridge)
• Low metal-ion leakage in eluted his-tagged protein
• For purification under native or denaturing conditions
• Fast and reproducible processing on any LC system, including ÄKTA

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Applications

Purified recombinant his-tagged proteins can be used for:

• Protein labeling
• Immunization to produce antibodies
• Animal studies
• Other applications

Additional products

His60 Ni resin (a nickel-based resin) is sold separately as:

• His60 Ni resins
• His60 Ni gravity columns

TALON resin (a cobalt-based resin) is sold separately as:

• TALON resins
• TALON gravity columns
• TALON FPLC cartridges

Detect his-tagged proteins with his tag antibodies

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.