Takara Selectable mammalian expression vectors

The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be confident that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest.

The pIRES selection vectors pIRESpuro3, pIREShyg3, pIRESneo3, and pIRESbleo3 each contain a multiple cloning site located upstream of a selection marker expressing IRES. Because your gene of interest and a selection marker are translated from a single RNA, you can be confident that nearly 100% of the colonies resistant to puromycin, hygromycin, G418, or bleomycin also express your protein of interest. All IRES selection vectors contain a partially disabled IRES which reduces the efficiency of translation initiation for the selection marker relative to that of the cloned gene and allows preferential selection of cells expressing high levels of your protein of interest.

The pBApo series of vectors are mammalian expression vectors for constitutive expression of your gene of interest from either a CMV or EF1-alpha promoter. The antibiotic resistance marker, either puromycin or neomycin, is expressed separately from an SV40 promoter. pBApo-CMV lacks a selectable marker.

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Overview

• pIRES vectors include either puromycin, hygromycin, G418, or bleomycin resistance for selection
• pBApo vectors include either neomycin or puromycin resistance for selection
• Allow for stable product expression in mammalian cells

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Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.