Takara CRISPR screen to determine host factors

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Examples of Studies Using Genome-wide CRISPR Screens for Virology

• Flint, M. A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus. Nat. Comm. 285, (2019).
• Gebre, M. et al. CRISPR-Cas9 Genetic Analysis of Virus-Host Interactions. Viruses 10, 55 (2018).
• Han, J. Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication. Cell Rep. 23, 596–607 (2018).
• Li, B. et al. Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection. Nat. Comm. 11, 164 (2020).
• Marceau, C. D. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535, 159–163 (2016).
• Park, R. J. A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Nat. Genet. 49, 193–203 (2017).
• Puschnik, A. S. et al. A CRISPR toolbox to study virus-host interactions. Nat. Rev. Microbiol. 15, 351–364 (2017).

References

• Doench, J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol. 34, 184–191 (2016).
• Doench, J. G. et al. Am I ready for CRISPR? A user’s guide to genetic screens. Nat. Rev. Genet. 19, 67–80 (2018).

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