Takara CRISPR screen to determine host factors

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Examples of studies using genome-wide CRISPR screens for virology

Flint, M. A genome-wide CRISPR screen identifies N-acetylglucosamine-1-phosphate transferase as a potential antiviral target for Ebola virus. Nat. Comm. 285, (2019).

Gebre, M. et al. CRISPR-Cas9 Genetic Analysis of Virus-Host Interactions. Viruses 10, 55 (2018).

Han, J. Genome-wide CRISPR/Cas9 Screen Identifies Host Factors Essential for Influenza Virus Replication. Cell Rep. 23, 596–607 (2018).

Li, B. et al. Genome-wide CRISPR screen identifies host dependency factors for influenza A virus infection. Nat. Comm. 11, 164 (2020).

Marceau, C. D. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535, 159–163 (2016).

Park, R. J. A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Nat. Genet. 49, 193–203 (2017).

Puschnik, A. S. et al. A CRISPR toolbox to study virus-host interactions. Nat. Rev. Microbiol. 15, 351–364 (2017).

References

Doench, J. G. et al. Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Nat. Biotechnol. 34, 184–191 (2016).

Doench, J. G. et al. Am I ready for CRISPR? A user`s guide to genetic screens. Nat. Rev. Genet. 19, 67–80 (2018).

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.

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