Thermo Fisher Scientific Robo3 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reconstitute in 100 µL of sterile water.
• Centrifuge to remove any insoluble material.
• Specificity: Robo3.

Target Information

The ROBO3 gene belongs to the Roundabout (ROBO) family, which regulates neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are part of the immunoglobulin transmembrane receptor superfamily. SLIT proteins (1–3) act as ligands for ROBO proteins, and SLIT/ROBO interactions influence processes such as myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, vasculogenesis, and neurogenesis.

ROBO3 encodes a protein with five immunoglobulin-like loops, three fibronectin type III motifs, a transmembrane segment, and a cytoplasmic tail containing conserved signaling motifs (CC0, CC2, CC3). Unlike other ROBO family members, ROBO3 lacks the CC1 motif. It plays a critical role in axonal navigation at the neural tube’s ventral midline. In mice, loss of Robo3 leads to failure of commissural axons to cross the midline in the spinal cord and hindbrain. Mutations in ROBO3 cause horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder characterized by congenital absence of horizontal gaze and progressive scoliosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.