Thermo Fisher Scientific AGO2 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.5 µg/mL

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Ago proteins are broadly expressed in somatic cells, associate with miRNAs, and are key actors in different RNA silencing pathways. Only the Ago2 protein displays endonucleolytic or “Slicer” activity and can execute miRNA-directed cleavage of target mRNA when base-pairing is perfect. In cases of partial complementarity, Ago2 interferes with translation via its repression activity.

Ago2 plays a non-redundant role in small RNA-guided gene silencing processes, including RNA interference, translation repression, and heterochromatinization. As a core element of the RISC complex, AGO2 initiates degradation of target mRNAs through catalytic activity guided by siRNAs or miRNAs. It also acts as an RNA slicer in a Dicer-independent manner and regulates miRNA maturation.

Over-expression of Ago2 has been correlated with tumor cell growth and cancer patient survival. Gene disruption in mice demonstrates that Ago2 is essential for embryonic development and regulates B-lymphoid and erythroid development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지

(이미지 파일: PA5-78738_AGO2_Q9UKV8-1_Rabbit.svg, PA5-78738_AGO2_Q9UKV8-1_Rabbit_PDP.jpeg)