Thermo Fisher Scientific CD86 Monoclonal Antibody (2B11)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

CD86, also known as B7-2, B70, and Ly-58, is a member of the immunoglobulin superfamily. It is expressed on B lymphocytes, T lymphocytes, dendritic cells, and astrocytes. As a ligand for CD28 and CD152 (CTLA-4), CD86 plays a critical role in T cell–B cell interactions. The interaction of CD86 with CD28 can transduce co-stimulatory signals for T cell proliferation and cytokine production. CD86 is also involved in NK cell-mediated cytotoxicity.

Human CD86 Antibody (2B11), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with CD86.

Target Information

CD86 is one of two ligands (the other being CD80) for CTLA4 and CD28. It acts as a costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation without sufficient co-stimulation involving CD86/CD80 can induce tolerance.

CD86 is a type I membrane protein and a member of the immunoglobulin superfamily. It is expressed by antigen-presenting cells and binds to two T-cell surface proteins: CD28 antigen and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4). Binding to CD28 provides a costimulatory signal for T-cell activation, while binding to CTLA-4 negatively regulates T-cell activation and diminishes the immune response.

Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.