Thermo Fisher Scientific CD86 Monoclonal Antibody (2B11)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1- 5 µg/mL

-

ELISA (ELISA)

0.01-0.05 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B11

Immunogen

Recombinant CD86 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

CD86, known as B7-2, B70, and Ly-58, is a member of immunoglobulin superfamily. It is expressed on the surface of B lymphocytes, T lymphocytes, dendritic cells and astrocytes. As a ligand for CD28 and CD152 (CTLA-4), CD86 plays a critical role in T cell-B cell interactions. The interaction of CD86 with CD28 can transduce co-stimulatory signals for T cell proliferation and cytokine production. CD86 is also involved in NK cell-mediated cytotoxicity.
Human CD86 Antibody (2B11), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with CD86.

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.