Thermo Fisher Scientific PKCgamma Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide (KLH-coupled) derived from human PKCγ

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Protein kinase C (PKC, EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. It is involved in diverse cellular signaling pathways. Specifically, PKC family members serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.PKC family is divided into three groups based on different enzymes cofactor requirements. They are conventional (c) PKC isoforms, novel (n) PKC isoforms, and atypical (a)PKC isoforms respectively. Conventional (c) PKC isoforms consisting α, βI, βII and γ require diacylglycerol (DAG), Ca2+, and phospholipid for activation. Novel (n)PKC isoforms consisting δ, ε, η (also known as PKC-L), θ and µ (the mouse homolog of human PKCµ, known as PKD) require DAG but not Ca2+ for activation. Atypical (a)PKC isoforms, consisting ζ, ι and λ (the mouse homolog of human PKC ι) require neither Ca2+ nor DAG. Other than those listed above, a new PKC member has recently been discovered and is referred to as PKCν. PKCγ (PKC gamma) plays a key role in neuronal signal transduction and regulating intercellular communication.GenScript Rabbit Anti-PKCγ Polyclonal Antibody is developed in rabbit using a synthetic peptide (KLH-coupled) derived from human PKCγ.

Target Information

The PKC family of serine/threonine kinases, including PRKCG (PKC gamma), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.