Thermo Fisher Scientific Phospho-Akt (Ser473) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2.0 µg/mL

-

ELISA (ELISA)

0.05-0.2 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthesized phosphopeptide (KLH conjugated) derived from human Akt around the phosphorylation site of serine 473 (Q-F-S473-Y-S) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AKT1,uniProtId:P31749-1,ncbiNodeId:9606,antigenRange:473,antigenLength:480,antigenImageFileName:A00959-40_AKT1_P31749-1_Rabbit.svg,antigenImageFileNamePDP:A00959-40_AKT1_P31749-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.

Akt, also known as protein kinase B (PKB), a serine/ threonine kinase, is a critical enzyme in several signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. Akt is activated following its phosphorylation at two regulatory residues. Phosphorylation of threonine on the kinase domain, catalyzed by PDK1, is essential for Akt activation. Akt activity is augmented approximately 10-fold by phosphorylation at the serine on the hydrophobic motif by PDK2. Phosphorylation of Thr308 and Ser473 activates Akt α. Phosphorylation at Thr309 and Ser474 on Akt β1 and β2, and on Thr305 on Akt γ result in their activation. Akt promotes cell survival by inhibiting apoptosis by phosphorylating and inactivating several targets, including Bad, forkhead transcription factors, c-Raf and caspase-9. The activation of Akt is negatively regulated by PTEN, a PIP3 specific phosphatase, and SHIP, a SH2-domain containing inositol 5-phosphatase.GenScript Rabbit Anti-Akt (Ser473) Polyclonal Antibody is developed in rabbit using a synthetic peptide corresponding to residues surrounding S473 of human, rat and mouse Akt α, also known as protein kinase B alpha (PKBα), Akt1, and RACα. In addition, this peptide is identical to the sequence surrounding S474 of human, rat and mouse Akt β (Akt2) and S472 of human and mouse Akt γ (Akt3).

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.