Thermo Fisher Scientific Calnexin Polyclonal Antibody

Applications and Tested Dilution

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Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL.

Calnexin is a calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum (ER). It assists in protein assembly and retention of unassembled subunits within the ER. Calnexin plays a major role in ER quality control by retaining incorrectly folded proteins.

This antibody was developed in rabbit hosts using a KLH-coupled synthetic peptide from the carboxyl terminal of human calnexin (Swiss Prot: P27824).

Target Information

Calnexin, also known as IP90, p88, or p90, is an approximately 90 kDa integral membrane protein of the endoplasmic reticulum. It acts as a molecular chaperone involved in proper folding and assembly of polypeptides into multisubunit complexes.

Calnexin associates with MHC class I heavy chains, partial complexes of T cell receptors and B cell membrane immunoglobulins, but not with completed receptor complexes. It retains incompletely or improperly folded proteins within the ER.

Soluble ER resident proteins often contain the KDEL sequence for ER retention, while integral membrane proteins like calnexin lack this motif but possess positively charged cytosolic residues ensuring ER localization.

Calnexin structure:

• ER luminal domain: 461 amino acids
• Transmembrane segment: 22 amino acids
• Cytoplasmic tail: 89 amino acids

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.