Takara Recombinant Ribonuclease Inhibitor, GPR

Overview

Recombinant Ribonuclease Inhibitor, GPR은 일반 목적 시약(GPR) 요구사항에 따라 제조 및 라벨링된 제품으로, RNase A 활성을 억제하여 RNA 안정성을 유지하는데 사용됩니다.

Applications

• cDNA synthesis (Ribonuclease Inhibitor: 0.5 U/µl reaction)
• In vitro translation (Ribonuclease Inhibitor: 1 U/µl reaction)
• In vitro transcription using cell-free extract (Ribonuclease Inhibitor: 20 U/µl reaction)
• In vitro transcription with SP6 or T7 RNA Polymerase (Ribonuclease Inhibitor: 1 U/µl reaction)
• Polysome isolation (Ribonuclease Inhibitor: 1,000 U/ml reaction)

Components

Source

• E. coli containing a plasmid that carries the porcine RNase Inhibitor gene

Storage

• –20°C

Concentration

• 40 U/µl

Unit Definition

One unit is defined as the amount of Recombinant Ribonuclease Inhibitor, GPR required to inhibit the activity of 5 ng of RNase A by 50%.
Activity is determined as the ability to inhibit hydrolysis of cytidine 2,3`-cyclic monophosphate (cyclic CMP) by RNAse A.

Product Citations

• Blackburn, P. J. Biol. Chem. 254, 12484–7 (1979).
• Blackburn, P., Wilson, G. & Moore, S. J. Biol. Chem. 252, 5904–10 (1977).
• Burton, L. E. & Fucci, N. P. Int. J. Pept. Protein Res. 19, 372–9 (1982).
• Eichler, D. C., Tatar, T. F. & Lasater, L. S. Biochem. Biophys. Res. Commun. 101, 396–403 (1981).
• de Martynoff, G., Pays, E. & Vassart, G. Biochem. Biophys. Res. Commun. 93, 645–53 (1980).
• Scheele, G. & Blackburn, P. Proc. Natl. Acad. Sci. U.S.A. 76, 4898–902 (1979).
• Turner, P. M., Lerea, K. M. & Kull, F. J. Biochem. Biophys. Res. Commun. 114, 1154–60 (1983).

Additional Product Information

Please refer to the product’s Certificate of Analysis for detailed information regarding storage conditions, product components, and technical specifications.
Kit Components Lists and Certificates of Analysis are available under the Documents tab.