Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12)
Phospho-ATM (Ser1981) 단클론 항체로 인간, 마우스, 랫트 시료에 반응. DNA 손상 반응 및 세포주기 조절 연구에 적합. Western blot, IHC, ICC/IF, IP 등 다양한 응용 가능. 단백질 G 정제, 액상 형태, 1 mg/mL 농도.
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:1,000 | - |
| Immunoprecipitation (IP) | 1:10–1:500 | - |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10H11.E12 |
| Immunogen | A synthetic peptide with a phosphorylated serine (1981) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2062982 |
Product Specific Information
- Does not react with canine samples.
- Suggested positive control: Irradiated normal human fibroblasts.
- No reactivity against un-irradiated cell extracts.
Target Information
Ataxia-telangiectasia Mutated (ATM) is a protein belonging to the PI3/PI4 kinase family.
Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and increased risk of lymphoid cancer.
The ATM gene encodes a protein in the phosphoinositide 3-kinase (PI3K) superfamily that phosphorylates proteins rather than lipids.
ATM targets several cell-cycle regulators including P53, Mdm2, BRCA1, and SMC1, and plays a key role in repairing double-stranded DNA breaks caused by ionizing radiation and mutagens.
ATM becomes autophosphorylated and activated upon exposure to ionizing radiation.
AT cells are hypersensitive to radiation, show impaired DNA synthesis inhibition, and delayed p53 induction.
DNA damage activates ATM kinase, triggering a cascade of reactions regulating cell cycle, apoptosis, and DNA repair.
ATM is also linked to apoptosis through interactions with Nbs1 and Chk2 in the E2F1 pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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