Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (pY20)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 46 publications 46 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 5 publications 5 publications

ELISA (ELISA)

100 ng/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

-

View 9 publications 9 publications

Miscellaneous PubMed (Misc)

-

View 20 publications 20 publications

Product Specifications

Species Reactivity

Chemical

Published species

Bovine, Chemical

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

pY20

Immunogen

Proprietary Immunogen.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

BBS, pH 8.0

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_935102

Product Specific Information

For the detection of phosphoproteins, tyrosine phosphatase inhibitors such as 1mM sodium orthovanadate should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.

Mouse anti Phosphotyrosine antibody, clone PY20 recognizes phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues.

Target Information

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.