Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 563, 661, 805
• Brilliant Violet 421, 650, 711
• eFluor 450, 506, 570, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The BM8 monoclonal antibody reacts with mouse F4/80 antigen (~160 kDa surface receptor) belonging to the EGF-TM7 family. It contains seven EGF-like domains, seven transmembrane segments, and a cytoplasmic domain homologous to TM7 superfamily members. F4/80 is expressed by most mature macrophages and serves as a key marker for this cell population. It may also be expressed on peritoneal eosinophils, Langerhans cells, and some dendritic cell subsets.

Expression begins early in myeloid development and can be upregulated by M-CSF stimulation. Some macrophage subsets, particularly in lymphoid tissues, may lack F4/80 expression.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on resident peritoneal macrophages; ≤1.0 µg/test recommended. Optimal titration is advised per assay.

Fluorochrome Information:
Super Bright 436 is excited by the violet laser (405 nm) and emits at 436 nm. Recommended detection filter: 450/50 bandpass or equivalent. Ensure instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein and a member of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies during macrophage maturation and activation and is found in Kupffer cells, Langerhans cells, microglia, and macrophages in spleen, lung, thymus, and bone marrow. Some dendritic cells also express F4/80, but it is absent in T-cell zone macrophages. F4/80 may play a role in generating efferent CD8⁺ regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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