Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright 436, eBioscience

Thermo Fisher Scientific MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), Super Bright 436, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 496, 661, 737
• Brilliant Violet 421, 605, 650, 711, 786
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue 510, 610-70S, 660-120S
• NovaFluor Red 685, 710
• NovaFluor Yellow 570, 590, 660
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600, 645, 702, 780
• Custom conjugation available

Product Specific Information

The M5/114.15.2 monoclonal antibody reacts with mouse MHC class II glycoproteins encoded by I-A and I-E subregions (I-A^b, I-A^d, I-A^q, I-E^d, I-E^k, not I-A^f, I-A^k, or I-A^s).
It detects polymorphic determinants on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying H-2^b, H-2^d, H-2^q, H-2^p, H-2^r, and H-2^u haplotypes, but not H-2^s or H-2^f.
M5/114 mAb inhibits I-A-restricted T cell responses in H-2^b, H-2^d, H-2^q, H-2^u haplotypes.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes. ≤0.125 µg/test recommended.
A test = antibody amount for staining a sample in 100 µL final volume (10⁵–10⁸ cells/test). Optimize titration for best results.

Fluorochrome characteristics:
Super Bright 436 excited with violet laser (405 nm), emits at 436 nm. Recommended filter: 450/50 bandpass or equivalent.
Ensure instrument compatibility.
For panels with multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.

Excitation: 405 nm
Emission: 436 nm
Laser: Violet

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

MHC class II molecules are transmembrane glycoproteins expressed on antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind endocytosed exogenous antigens and present them to T cells.
The antigen-binding groove between MHC class II α and β chains is open at both ends and accommodates peptides 15–24 amino acids long.
HLA and MHC antibodies are essential tools in immunopeptidomics, enabling neoantigen identification via LC-MS/MS analysis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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