Thermo Fisher Scientific Human BMP-4 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

In vitro Assay (IV)

-

View 125 publications 125 publications

Miscellaneous PubMed (Misc)

-

View 12 publications 12 publications

Product Specifications

Species

Human

Published species

Avian, Bovine, Human, Mouse, Pig, Rat, Rhesus monkey

Expression System

HeLa cells

Amino acid sequence

HHSQRARKKN KNCRRHSLYV DFSDVGWNDW IVAPPGYQAF YCHGDCPFPL ADHLNSTNHA IVQTLVNSVN SSIPKACCVP TELSAISMLY LDEYDKVVLK NYQEMVVEGC GCR

Molecular weight

25.6 kDa

Class

Recombinant

Type

Protein

Purity

≥ 97% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 2-8 ng/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Product Specific Information

120-05-1MG will be provided as 10 x 100 µg (120-05-100UG).

120-05-500UG will be provided as 5 x 100 µg (120-05-100UG).

Recombinant Human BMP-4, expressed in HeLa cells, is a 25.6 kDa homodimeric glycoprotein.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5` untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.