Thermo Fisher Scientific CD5 Monoclonal Antibody (UCHT2), PE, eBioscience
인간 CD5 단백질을 인식하는 UCHT2 클론의 마우스 단일클론 항체로, PE 형광표지되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, 5 µL/test로 사용 가능합니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT2 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1548712 |
Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 660-120S
- NovaFluor Red 710
- NovaFluor Yellow 660
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Custom conjugation available
Product Specific Information
The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by most thymocytes, mature T cells, and a subset of B cells. CD5 signaling activates T cells and mediates T-B cell interactions via binding to CD72 on B cells.
The UCHT2 clone recognizes primate CD5.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and tested for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.5 µg) per 100 µL test volume. Suitable for 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD5 is a 67 kDa single-chain transmembrane glycoprotein present on all mature T-lymphocytes, most thymocytes, and many T-cell leukemias and lymphomas. It also reacts with a subset of activated B cells and may regulate T-cell proliferation. CD5 is found on 95% of thymocytes and about 72% of peripheral blood lymphocytes. In lymph nodes, reactivity is mainly in T cell areas.
Diseases associated with CD5 dysfunction include thymus cancer and Richter's Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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