Thermo Fisher Scientific Oncomine BRCA Research Assay, Chef-Ready Library Preparation

Oncomine™ BRCA Research Assay, Chef-Ready Library Preparation

The Oncomine™ BRCA Research Assay consists of two pools of AmpliSeq™ oligonucleotide primers and associated reagents to generate amplicon libraries for next-generation sequencing (NGS) on Ion Torrent™ platforms.
This assay provides sensitive and comprehensive amplification of all coding regions of the human BRCA1 and BRCA2 genes.
This version is optimized for automated library preparation using the Ion Chef™ System.
For manual library preparation, refer to Cat. No. A32840.

주요 특징

• Validated for use on Ion 318 and Ion 530 chips
• Detects SNVs, InDels, and large exon/gene deletions or duplications
• Compatible with as little as 10 ng input DNA from FFPE tissue or blood samples
• Highly uniform coverage across all coding exons & splice sites for efficient sequencing and accurate analysis
• Optimized primer design and amplification chemistry for specific target enrichment
• Validated detection of somatic variants to 5% and lower
• Simple and fast workflow producing targeted libraries in ~3.5 hours

제품 개요

The Oncomine BRCA Research Assay is a complete kit facilitating amplification of the entire exonic region of both BRCA genes from FFPE tissue or blood samples with minimal input DNA.
Leveraging Ion AmpliSeq™ technology, this multiplexed NGS assay enables generation of results from multiple samples in a single run.
Designed for use on Ion PGM™ or Ion S5™ sequencing systems, results are typically delivered in days rather than weeks.

The assay integrates with Torrent Suite™ and Ion Reporter™ software for optimized variant calling of SNV, InDel, and large exon/gene deletion/duplication detection, supporting both germline and somatic DNA analysis workflows.

BRCA 유전자 정보

BRCA1 and BRCA2 are tumor suppressor genes involved in the homologous recombination pathway of DNA damage repair.
Mutations in these genes can impair repair mechanisms and are linked to hereditary cancer predisposition.
Such errors are found in both germline and somatic mutations, as well as in tumor samples.

사양 (Specifications)

제품 이미지

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