Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), eBioscience
마우스 Granzyme B를 인식하는 Rat IgG2b 단일클론 항체로, 세포독성 T세포 및 NK세포의 GrB 검출에 적합합니다. Western blot, IHC, ELISA 등 다양한 응용에 사용 가능하며, 높은 순도(>90%)와 낮은 응집도(<10%)를 보유합니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Granzyme B Monoclonal Antibody (16G6), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | 4 publications |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent | - |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Flow Cytometry (Flow) | - | 14 publications |
| ELISA | 1–4 µg/mL | - |
| Functional Assay | - | 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 16G6 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865289 |
| Purity | >90% (SDS-PAGE) |
| Aggregation | <10% (HPLC) |
| Filtration | 0.2 µm post-manufacturing filtered |
Product Specific Information
The 16G6 antibody reacts with mouse Granzyme B (GrB), a member of the granzyme serine protease family found in cytotoxic T cells and NK cells. Granzyme B (also known as CGL1 or CTLA-1) is crucial for inducing apoptosis in target cells via interaction with cytotoxic T cells. The mannose 6-phosphate receptor acts as a death receptor for Granzyme B during this process.
For intracellular staining and flow cytometric analysis using direct conjugates of anti-mouse Granzyme B, it is recommended to use the Foxp3 Staining Buffer Set (Product # 00-5523).
Applications Reported:
The 16G6 antibody has been used for western blotting, immunohistochemical staining, ELISA capture, and staining of formalin-fixed paraffin-embedded tissue sections.
Applications Tested:
- Sandwich ELISA for mouse Granzyme B quantitation (capture antibody, 1–4 µg/mL)
- Western blotting (recommended starting concentration: 1 µg/mL)
- Formalin-fixed paraffin-embedded tissue (≤20 µg/mL, low pH antigen retrieval)
Careful titration is recommended for optimal assay performance.
Target Information
Granzyme B is a serine protease found in cytotoxic T cells and NK cells. It induces apoptosis through caspase-3 activation and mitochondrial pathways (via Bid cleavage). Granzymes (A–G) are stored in lytic granules of CTLs and NK cells. Granzyme B is essential for immune-mediated cytotoxicity and apoptosis induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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