Thermo Fisher Scientific SSEA4 Monoclonal Antibody (eBioMC-813-70 (MC-813-70)), eBioscience
SSEA-4 항원을 인식하는 마우스 단클론 항체로, 인간 및 다양한 종의 줄기세포 표지 연구에 사용됩니다. Flow cytometry, IHC, ICC/IF에 적합하며, 고순도(>90%)로 정제된 액상 형태입니다. 4°C에서 보관하며 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent | 1 publication |
| Immunocytochemistry (ICC/IF) | Assay-Dependent | 2 publications |
| Flow Cytometry (Flow) | 1 µg/test | 9 publications |
| Miscellaneous PubMed (Misc) | - | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Chicken, Human, Mouse, Non-human primate, Rabbit |
| Published Species | Human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioMC-813-70 (MC-813-70) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865293 |
Additional Formats
- Alexa Fluor 488
- Biotin
- eFluor 660
- PE
- PerCP-eFluor 710
- Custom conjugation available upon request
Product Specific Information
The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), embryonic germ cells (EG), induced pluripotent stem (iPS) cells, and embryonic stem cells (ES), with decreased expression upon differentiation. Expression has also been observed on bone marrow mesenchymal stem cells.
This antibody cross-reacts with mouse, rabbit, canine, chicken, and Old/New World monkeys.
Applications Reported: Flow cytometry, immunohistochemistry, immunocytochemistry.
Applications Tested: Flow cytometric analysis of 2102Ep cell line, ≤1 µg/test (in 100 µL volume, 10⁵–10⁸ cells/test). Optimal titration recommended.
Purity: >90% by SDS-PAGE
Aggregation: <10% by HPLC
Filtration: 0.2 µm post-manufacturing filtered
Target Information
SSEA-4 (Stage-specific embryonic antigen 4) is a cell surface antigen expressed along with SSEA-3, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these markers are down-regulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-induced differentiation. In mouse stem cells, SSEA-1 expression decreases with differentiation, while SSEA-3 and SSEA-4 expression increase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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