Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), eBioscience

Thermo Fisher Scientific SSEA3 Monoclonal Antibody (eBioMC-631 (MC-631)), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats:

• Alexa Fluor 488
• Biotin
• PE
• Request custom conjugation

Product Specific Information

The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG), and human embryonic stem cells (ES), and is downregulated following differentiation.

Applications Reported:
This antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry.

Applications Tested:
Tested by flow cytometric analysis of the 2102Ep cell line. Recommended usage ≤ 0.5 µg/test (100 µL final volume). Cell number may range from 10⁵ to 10⁸ cells/test. Antibody titration is recommended for optimal performance.

Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

SSEA-3 (Stage-specific embryonic antigen 3) is a cell surface antigen expressed with SSEA-4, TRA-1-60, and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are downregulated during differentiation, while SSEA-1 is absent in undifferentiated human stem cells and upregulated after retinoic acid-mediated differentiation. In mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.