Thermo Fisher Scientific CD68 Monoclonal Antibody (KP1), eBioscience

Thermo Fisher Scientific CD68 Monoclonal Antibody (KP1), eBioscience

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Description:
The KP1 antibody reacts with human CD68, a member of the sialomucin family related to lysosomal-associated membrane proteins (LAMPs) and scavenger receptors. CD68 is primarily an intracellular protein located in the late endosomal compartment, with minor expression on the surface of myeloid-derived cells such as monocytes, macrophages, dendritic cells, neutrophils, basophils, mast cells, and CD34+ progenitor cells. It may play roles in antigen processing/presentation and lysosomal membrane protection. Expression has also been observed in activated T cells and subsets of B cells and fibroblasts.

Applications Reported:
Validated for flow cytometry, immunoprecipitation, western blotting, and immunohistochemistry (frozen and FFPE tissue sections).

Applications Tested:
Tested by IHC with both low and high pH antigen retrieval on FFPE tissue. Recommended concentration ≤ 1 µg/mL. Optimize dilution per assay.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein primarily expressed in lysosomes of monocytes and macrophages, and to a lesser extent in dendritic cells and granulocytes. It is involved in phagocytic activity, lysosomal metabolism, and cell-pathogen interactions. CD68 expression is noted in interdigitating reticulum cells, histiocytic lymphoma, AML, and granulocytic sarcoma. Elevated CD68 levels have been found in CD34+ cells in various malignancies.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.