Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (FN50), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugate Formats

• Alexa Fluor 561
• Alexa Fluor 660
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395, 615, 661, 737, 805
• Brilliant Violet 421, 480, 650, 711, 786
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 780
• Request custom conjugation

Product Specific Information

The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 (~30 kDa) is expressed as a disulfide-linked dimer on the cell surface and is rapidly upregulated upon activation. It is found on lymphocytes, monocytes, and platelets.

Applications Reported:
Flow cytometric analysis and immunohistochemical staining.

Applications Tested:
Tested by flow cytometric analysis of resting and 6-hour TPA-activated human peripheral blood cells. Recommended ≤0.5 µg per test (defined as staining a cell sample in 100 µL). Cell number may range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa under non-reducing conditions). It contains one or two N-linked oligosaccharides and is present on activated platelets. In normal peripheral blood, a variable percentage of cells express CD69, which is involved in lymphocyte signal transduction. CD69 expression is induced upon activation of T lymphocytes and may play a role in proliferation. It also transmits signals in NK cells and platelets. Alternative splicing results in multiple transcript variants. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.