Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), eBioscience

Thermo Fisher Scientific CD69 Monoclonal Antibody (H1.2F3), eBioscience

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Custom conjugation available

Product Specific Information

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and expressed on the surface as a disulfide-linked dimer. Activation of T and B cells rapidly upregulates CD69 expression, indicating its role in lymphocyte development and activation.

Applications Reported:

• Flow cytometric analysis
• Immunoprecipitation
• In vitro cell activation (use Functional Grade purified H1.2F3, Product #16-0691-85 for functional assays)

Applications Tested:
Tested by flow cytometric analysis of resting and activated mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume). Cell number may vary from 10⁵–10⁸ cells/test. Titrate for optimal performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide-bonded homodimer (~60 kDa, non-reducing conditions). It contains one or two N-linked oligosaccharides and is expressed on activated platelets. CD69 plays a role in lymphocyte signal transduction and proliferation, and may transmit signals in NK cells and platelets. Alternative splicing results in multiple transcript variants. CD69 dysfunction is associated with diseases such as coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.