Thermo Fisher Scientific tPA Monoclonal Antibody (OTI3H3)
tPA 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI3H3). Western blot과 IHC(P)에서 검증됨. Human 시료에 반응하며, 비결합(Unconjugated) 형태로 제공. 액상 형태, 1 mg/mL 농도, -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 157–426 of PLAT produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725542 |
Target Information
Tissue plasminogen activator (tPA) converts the inactive zymogen plasminogen into plasmin by hydrolyzing a single Arg-Val bond. Through regulation of plasmin-mediated proteolysis, it plays a critical role in tissue remodeling, degradation, and cell migration.
tPA is a heterodimer of chain A and chain B linked by a disulfide bond and binds fibrin with high affinity, enhancing catalytic efficiency 100–1000 fold. It also interacts with heparin, laminin, fibronectin, mannose receptor, and LRP1, which are involved in clearance.
Binding to endothelial and vascular smooth muscle cells enhances plasminogen activation and reduces inhibition by PAI-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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