Thermo Fisher Scientific Human FXR, Ligand Binding Domain, GST Tag Recombinant Protein

Thermo Fisher Scientific Human FXR, Ligand Binding Domain, GST Tag Recombinant Protein

Applications

• Ligand Binding Assay (LBA)

Tested Dilution

• Assay-dependent

Product Specifications

Product Specific Information

Farnesoid X Receptor, Ligand Binding Domain (FXR-LBD) is a 60.07 kDa recombinant human protein (amino acids 193–472) expressed as a GST fusion protein in baculovirus-infected insect cells.
FXR-LBD was expressed and purified in the absence of exogenous ligands, making it ideal for ligand binding and coregulator displacement studies.

Protein Form: Recombinant, Ligand Binding Domain

Target Information

FXR (farnesoid X receptor; NR1H4) belongs to the nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid, and deoxycholic acid.
FXR plays an essential role in bile acid homeostasis through regulation of genes involved in bile acid synthesis, conjugation, and enterohepatic circulation. It also regulates lipid and glucose homeostasis and is involved in the innate immune response.

The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3', spaced by one nucleotide (IR-1), and also to tandem repeat DR1 sites with lower affinity.
In the liver, FXR activates transcription of the corepressor NR0B2, thereby indirectly inhibiting CYP7A1 and CYP8B1 (involved in bile acid synthesis). It also regulates SLC10A1/NTCP (hepatic uptake of conjugated bile acids), SLC27A5/BACS and BAAT (bile acid conjugation), ABCB11/BSEP (bile salt export), ABCC2/MRP2 (secretion of conjugated bile acids), and ABCB4 (phosphatidylcholine secretion in the small intestine).

(From Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.