LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit, goat

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15142:The LanthaScreen™ TR-FRET PXR (SXR) Competitive자세히 알아보기

This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15142:

The LanthaScreen™ TR-FRET PXR (SXR) Competitive Binding Assay Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for the pregnane X receptor (PXR), also known as the steroid and xenobiotic receptor (SXR). The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescent small-molecule PXR ligand (Fluormone™ PXR (SXR) Green), and a human PXR (SXR) ligand-binding domain that is tagged with glutathione S-transferase (GST) in a homogeneous mix-and-read assay format.

*To assay:
*When running the LanthaScreen™ TR-FRET PXR (SXR) competitive binding assay, a Tb-labeled anti-GST antibody is used to indirectly label the receptor by binding to its GST tag. Competitive ligand binding is detected by a test compound`s ability to displace a fluorescent ligand (tracer) from the receptor, which results in a loss of FRET signal between the Tb-anti-GST antibody and the tracer (Figure 1). This type of binding assay is analogous to radioligand-based assays, except that it eliminates handling of radioactivity and enables a homogeneous, “addition-only ” format.

사양

콘주게이트Tb (Terbium)

검출 방법Fluorescence

용도(장비)Microplate Reader

수량800 x 20 μL assays

포장384-well Plate

어세이 입력Biochemical competitive binding

유전자 ID(Entrez)8856

판독End Point

배송 조건Dry Ice

타겟 입력NR1I2, PXR(SXR)

제품라인LanthaScreen™

용도(애플리케이션)Competitive Binding Assay, TR-FRET

리간드PXR

제품 유형TR-FRET PXR (SXR) Competitive Binding Assay Kit

No. of Assays800 x 20 μL assays

Unit SizeEach