Thermo Fisher Scientific Phospho-CDK1 (Tyr15) Polyclonal Antibody
Phospho-CDK1 (Tyr15) 폴리클로날 항체로 인간 CDK1 인산화 잔기를 특이적으로 인식합니다. Western blot, IHC, IP에 적합하며, 항원 친화 크로마토그래피로 정제된 고순도 제품입니다. 세포주기 조절 및 CDK1 관련 연구에 활용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human CDC2 (phospho Tyr15) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.84 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792648 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Japanese Medaka (100%), Drosophila (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
- Positive Controls: HeLa (untreated), HeLa (2 mM double thymidine treatment and recovery for 3.5 hr), HeLa (2 mM double thymidine treatment and recovery for 12 hr), HeLa (100 ng/mL Nocodazole treatment for 24 hr), Rat-2 (untreated), Rat-2 (100 ng/mL Nocodazole treatment for 24 hr).
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CDK1 (Cell Division Control protein 1) is essential for the completion of START, the controlling event in the cell cycle required to initiate mitosis.
CDK1 acts as a catalytic subunit of the M-Phase Promoting Factor, which induces entry into mitosis and is conserved across eukaryotes.
Phosphorylation of Bcl-2 in G2/M phase-arrested cells following photodynamic therapy with hypericin involves a CDK1-mediated signal and delays apoptosis onset.
Therapeutic potential of CDK inhibitor NU2058 in androgen-independent prostate cancer has also been demonstrated.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm. For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
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