Thermo Fisher Scientific ATPIF1 Polyclonal Antibody
ATPIF1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, ICC/IF, ELISA, IP 등에 사용 가능. 인간 및 마우스 반응성. 미토콘드리아 ATP 합성효소 복합체 연구에 적합. -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 26–106 of human ATPIF1 (NP_057395.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.93 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806444 |
Product Specific Information
- Immunogen sequence:
GSDQSENVDR GAGSIREAGG AFGKREQAEE ERYFRAQSRE QLAALKKHHE EEIVHHKKEI ERLQKEIERH KQKIKMLKHD D - Positive Samples: MCF-7, 22Rv1, HeLa, H460, Mouse brain
- Cellular Location: Mitochondrion
Target Information
Complex V, also called F1F0 ATPase or ATP synthase, is a highly conserved enzyme found in plants, fungi, bacteria, and animals. It catalyzes the reversible conversion of ADP and phosphate to ATP during oxidative phosphorylation, driven by proton flux across membranes.
Although traditionally localized to mitochondria, ATP synthase has also been identified on plasma membranes of hepatocytes (as HDL receptor), endothelial cells (as angiostatin receptor), and cancer cells.
The enzyme comprises two main sections:
- F1 ATP-ase (soluble): α, β, γ, δ, ε subunits
- F0 ATP-ase (membrane embedded): a, b, c subunits
In mammals, Complex V includes 17 subunits, with two encoded by mitochondrial DNA and the remainder by nuclear DNA. Mutations in mitochondrial-encoded subunits are associated with OXPHOS disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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