Thermo Fisher Scientific SMAD2 Polyclonal Antibody
SMAD2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 다양한 종(Human, Mouse, Rat)에 반응하며 WB, IHC, ELISA 등 여러 응용 분야에 사용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체로 연구용에 적합.
- 카탈로그번호
- 511300
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific SMAD2 Polyclonal Antibody
Thermo Fisher Scientific SMAD2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–3 µg/mL | 54 publications |
| Immunohistochemistry (IHC) | – | 5 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20–1:200 | – |
| Immunohistochemistry (Frozen) (IHC (F)) | – | 1 publication |
| Immunocytochemistry (ICC/IF) | – | 5 publications |
| ELISA | 0.1–1.0 µg/mL | 1 publication |
| Immunoprecipitation (IP) | – | 3 publications |
| ChIP assay (ChIP) | 1–3 µg × 10⁶ cells | 6 publications |
| Miscellaneous PubMed (Misc) | – | 4 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Dog, Human, Luciferase, Mouse, Non-human primate, Rat, Tag, Virus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | 27 amino acid synthetic peptide derived from the MH1 domain of human Smad2. Shares 24/27 residues with mouse Smad2 and 26/27 residues with Xenopus Smad2. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533896 |
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes such as cell proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors via SMAD anchor for receptor activation, playing a central role in TGF-beta signaling. Phosphorylation of threonine 8 by ERK1 enhances SMAD2 transcriptional activity, negatively regulated by calmodulin. Upon TGF-beta stimulation, SMAD2 is phosphorylated, dissociates from SARA, and associates with SMAD4, enabling nuclear translocation and transcriptional repression. SMAD2 can also be phosphorylated by activin type 1 receptor kinase and mediates activin signaling. Alternative splicing variants encoding SMAD2 have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.