Thermo Fisher Scientific beta Amyloid Polyclonal Antibody (CT695)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-2 µg/mL

View 47 publications 47 publications

Immunohistochemistry (IHC)

0.1-1.0 µg/mL

View 57 publications 57 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 8 publications 8 publications

Immunohistochemistry - Free Floating (IHC (Free))

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

-

View 7 publications 7 publications

ELISA (ELISA)

0.1-1.0 µg/mL

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 7 publications 7 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Hamster, Human, Mouse, Pig, Rat, Tag

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Clone

CT695

Immunogen

A 22 amino acid synthetic peptide derived from the C-terminus of the human beta-amyloid precursor protein (beta-APP). This sequence is 100% conserved in human, monkey, mouse, rat, guinea pig, and chicken beta-APP proteins. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Amyloid,uniProtId:P12023-1,ncbiNodeId:10090,antigenRange:770,antigenLength:770,antigenImageFileName:51-2700_beta_Amyloid_P12023-1_Rabbit.svg,antigenImageFileNamePDP:51-2700_beta_Amyloid_P12023-1_Rabbit_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533902

Product Specific Information

This antibody can be used to specifically detect the beta-amyloid precursor protein. The antibody reacts with full-length (APP695, 751, 770) and N-terminal truncated forms of beta-APP. The antibody can also be used to detect the C-terminal membrane-anchored fragment of beta-APP that remains after alpha- or beta-secretase cleavage. This antibody does not detect the beta-APP product N-terminal to the gamma-secretase cleavage site.

Reactivity with this antibody has been confirmed for human, mouse, pig, and rat. Based on sequence homology, reactivity with other species, including monkey, guinea pig, and chicken beta-APP, is highly likely. The CT695 antibody has demonstrated superiority in detecting axonal damage.

This antibody has been tested in western blotting with FRTL-5 (rat thyroid) cells and rat brain lysates. For immunohistochemistry FFPE tissues will require epitope retrieval pretreatment.

Target Information

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimers disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimers disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down`s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.