Thermo Fisher Scientific KCNH2 (HERG) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Reconstitution: Reconstitute with 25 µL, 50 µL, or 0.2 mL double distilled water (DDW), depending on sample size.
• Ships as a lyophilized powder at room temperature.
• Store at -20°C upon arrival.
• Reconstituted solution can be stored at 4°C for up to 1 week; for longer periods, store aliquots at -20°C.
• Avoid multiple freeze/thaw cycles.
• Centrifuge all antibody preparations before use (10,000 × g, 5 min).

Target Information

The human ether-a-go-go related gene (HERG) encodes the pore-forming alpha subunit of the delayed rectifier potassium channel IKr. There are two N-terminal splice variants:

• Isoform 1 alpha (full-length)
• Isoform 1 beta (shorter, lacking PAS motif)

Isoform 1 beta deactivates faster than isoform 1 alpha.
Residues within the C-terminal region influence channel expression and gating, including voltage-dependent activation.
HERG is expressed predominantly in the heart, especially in the ventricles.
Mutations in HERG can lead to increased beat-to-beat variability and early after-depolarizations, contributing to long QT syndrome type 2 and short QT syndrome type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.