Thermo Fisher Scientific Factor VIII Recombinant Rabbit Monoclonal Antibody (402)

Applications

ELISA

• Tested Dilution: 1:5,000–1:10,000

Product Specifications

Additional Formats

• Biotin
• HRP
• Request custom conjugation

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select optimal candidates for production.

Advantages of recombinant rabbit monoclonal antibodies:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to larger rabbit immune repertoire

This antibody has specificity for Human Coagulation Factor VIII/FVIII/F8 Light Chain.

Target Information

The F8 gene encodes coagulation factor VIII, a key component of the intrinsic pathway of blood coagulation. Factor VIII acts as a cofactor for factor IXa, which, in the presence of Ca²⁺ and phospholipids, converts factor X to its activated form Xa.

This gene produces two alternatively spliced transcripts:

1. Transcript variant 1 encodes a large glycoprotein (isoform a) that circulates in plasma and associates noncovalently with von Willebrand factor.
2. Transcript variant 2 encodes a smaller protein (isoform b) consisting primarily of the phospholipid-binding domain essential for coagulant activity.

Defects in this gene result in hemophilia A, a common recessive X-linked coagulation disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.