Thermo Fisher Scientific CRX Polyclonal Antibody
CRX 단백질을 인식하는 Sheep 유래 Polyclonal 항체로, Western blot 및 Immunocytochemistry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공. 인간 시료 반응성. 시각 관련 유전자 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E. coli-derived recombinant human CRX (Ala166–Leu299) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610298 |
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.
Target Information
The cone-rod homeobox-containing gene (CRX) encodes a transcription factor that coordinates the expression of several photoreceptor genes in the developing retina, including opsin and rhodopsin. CRX binds the OTX motif (TAATCC/A) upstream from photoreceptor genes.
The CRX gene is also expressed in pinealocytes of the pineal gland and may regulate pineal circadian activity by controlling melatonin synthesis genes.
CRX-deficient mice show disruption of circadian rhythms. The human CRX gene maps to chromosome 19q13.3, within the cone-rod dystrophy-2 locus (CORD2).
Mutations in CRX are implicated in visual pathologies such as CORD, Leber congenital amaurosis (LCA), and retinitis pigmentosa (RP).
All known CRX mutations produce disease in heterozygotes, though no correlation exists between phenotype and mutation type. Missense mutations affect the homeobox domain, while frameshift mutations affect the OTX domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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