Thermo Fisher Scientific MHC Class II Monoclonal Antibody (HIS19), Biotin, eBioscience

Applications

• Flow Cytometry (Flow): Assay-dependent
• Tested Dilution: Assay-Dependent
• Publications: View 1 publication

Product Specifications

Additional Formats

• Unconjugated
• APC
• FITC
• PE
• Request custom conjugation

Product Specific Information

The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except RT1n (including BN and MAXX strains).
Rat MHC class II (I-A equivalent), RT1B, is expressed by antigen-presenting cells such as B cells, dendritic cells, and macrophages.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on rat spleen or lymph node cell suspensions. Titrate for optimal performance.
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

MHC (Major Histocompatibility Complex) class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind endocytosed antigens and present them to T cells through an open-ended groove between the alpha and beta chains (15–24 amino acids long).
HLA and MHC antibodies are key in Immunopeptidomics, aiding neoantigen identification via HPLC-MS/MS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.