Thermo Fisher Scientific MHC Class II Monoclonal Antibody (HIS19), Biotin, eBioscience
쥐 MHC Class II 분자 RT1B를 인식하는 HIS19 단클론 항체입니다. Flow Cytometry용으로 검증되었으며, Biotin으로 표지되어 있습니다. 항원제시세포 분석에 적합하며, 4°C에서 암소 보관합니다.
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Applications
- Flow Cytometry (Flow): Assay-dependent
- Tested Dilution: Assay-Dependent
- Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIS19 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466538 |
Additional Formats
Product Specific Information
The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except RT1n (including BN and MAXX strains).
Rat MHC class II (I-A equivalent), RT1B, is expressed by antigen-presenting cells such as B cells, dendritic cells, and macrophages.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Tested on rat spleen or lymph node cell suspensions. Titrate for optimal performance.
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
MHC (Major Histocompatibility Complex) class II molecules are transmembrane glycoproteins expressed on professional antigen-presenting cells (macrophages, dendritic cells, B cells).
They bind endocytosed antigens and present them to T cells through an open-ended groove between the alpha and beta chains (15–24 amino acids long).
HLA and MHC antibodies are key in Immunopeptidomics, aiding neoantigen identification via HPLC-MS/MS.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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