Thermo Fisher Scientific Connexin 43 Monoclonal Antibody (Connexin43 (1A)), Biotin, eBioscience

Thermo Fisher Scientific Connexin 43 Monoclonal Antibody (Connexin43 (1A)), Biotin, eBioscience™

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

The Connexin43 monoclonal antibody recognizes mouse Connexin 43, a member of the connexin family of integral membrane proteins forming gap junction channels for direct cell-cell communication. Gap junctions consist of two hemichannels (connexons), each composed of six connexin proteins. Over 20 connexins have been identified, forming homomeric or heteromeric channels with distinct properties.

Connexin 43 is widely expressed in tissues including the central nervous system, heart, and bone. It is the predominant connexin in myocardium and astrocytes. Mutations in Connexin 43 are associated with oculodentodigital dysplasia, a developmental disorder with craniofacial and limb abnormalities. Connexin 43 is also a potential therapeutic target for malignant melanomas and mammary gland tumors.

This antibody recognizes mouse, human, and rat Connexin 43.
Applications reported include immunocytochemistry and immunohistochemistry of frozen tissue sections.
Tested for IHC(F) on frozen mouse tissue at ≤10 µg/mL; optimal concentration should be titrated for specific assays.

Target Information

Connexin 43 (Cx43) is a gap junction protein that forms hexameric hemichannels, enabling direct intercellular communication. These channels cluster to form gap junctions essential for development and cell growth regulation.

Phosphorylation of Cx43, particularly at Ser368 by PKC, modulates assembly and communication efficiency. Src kinase also phosphorylates Cx43, altering gap junction function. GFAP proteins facilitate ion and molecule transfer between cells. Gap junction proteins are classified into alpha and beta types based on sequence similarity.

Connexin 43 is the major gap junction protein in the heart, crucial for synchronized cardiac contraction and embryonic development. It is regulated by multiple kinases affecting myocardial coupling. A pseudogene (GJA1P) is mapped to chromosome 5. Mutations in GFAP are linked to X-linked Charcot-Marie-Tooth disease, oculodentodigital dysplasia, and heart malformations. Several alternatively spliced GFAP transcript variants exist.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.