Octet R4 Protein Analysis System

Highly Sensitive, Upgradeable System for Biomolecule Characterization

The Octet® R4 System Makes Biologics Development Affordable and Flexible

The 4-channel Octet® R4 instrument supports a wide range of analyses, including:

• Kinetic analysis
• IgG and protein titer measurement
• Reagent qualification
• Immunoassay development
• Bioprocess and quality analysis
• Crude antibody screening
• Epitope binning/mapping
• Ligand binding assays
• Small molecule analysis
• Cell signaling mechanism studies
• Infectious disease monitoring

Analysis can be performed using one to four channels, providing flexible sample throughput.
The Octet® R4 System offers a balance between throughput and cost, providing higher throughput than the 2-channel Octet® R2 and the same sensitivity as the 8-channel Octet® R8 at a lower price.

The Benefits of the Octet® R4 Protein Analysis System

Fast Results
Four parallel, independent channels enable increased flexibility and faster results.

High Sensitivity, Moderate Throughput, Low Cost
Ideal for moderate throughput sample analysis without compromising assay sensitivity.

Reduced Assay Time and Maintenance Cost
Fluidics-free Dip and Read format delivers faster results and lower process costs.
Crude sample compatibility eliminates pre-treatment, and non-destructive testing conserves samples.

Field Upgradeable within a Single Visit
Easily upgrade to an 8-channel Octet® system for higher throughput and GMP compliance.
Upgrades are completed on-site by Sartorius engineers in a single visit.

Sample Plate Cooling for Temperature-Sensitive Proteins
Temperature control from 15–40°C enables reliable kinetic determinations for temperature-sensitive proteins.
Rapid determination of binding rate constants at multiple temperatures allows extrapolation of thermodynamic data.
Four channels can be used independently or paired for high-quality kinetic characterization.

Applications

Kinetic and Affinity Characterization

Monitors binding events in real time to calculate:

• On rates (ka)
• Off rates (kd)
• Affinity constants (KD)

The detection surface directly contacts the sample, eliminating microfluidics.
Temperature control (15–40°C) ensures accurate kinetic determinations and thermodynamic extrapolation.

Protein Quantitation in Bioprocessing

Accurate quantitation of antibodies and proteins is essential for cell line selection and process optimization.
The Octet® R4 system measures specific proteins in solution with minimal matrix interference.
Concentrations are determined in as little as 2 minutes per sample using a simple one-step assay.
Rapid results improve process efficiency compared to HPLC or ELISA methods.

Technical Specifications

제품 이미지