Thermo Fisher Scientific TNFR1 Monoclonal Antibody (HM104)

Thermo Fisher Scientific TNFR1 Monoclonal Antibody (HM104)

Applications and Tested Dilution

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Product Specifications

Product Specific Information

The monoclonal antibody HM104 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of both membrane-bound and soluble forms. TNF-RI (~55–60 kDa) is expressed on most cell types and plays a key role in TNF-alpha–mediated cell stimulation. TNF-alpha triggers inflammatory responses, apoptosis, cellular proliferation, and may contribute to cancer progression. These effects are mediated through TNF-RI and TNF-RII, which share overlapping but distinct signaling pathways.

TNF-RI is activated by both soluble (17 kDa) and membrane-bound (26 kDa) TNF-alpha, while TNF-RII responds mainly to the membrane-bound form. Upon ligand binding, TNF-RI trimerizes and recruits a signaling complex including TRADD, RIP, cIAP1, TRAF2, and possibly TRAF5. Studies with TNF-RI-deficient mice demonstrate its major role in proliferation, inflammation, and apoptosis signaling.

Target Information

TNFR1 (Tumor Necrosis Factor Receptor 1) is a member of the TNF receptor superfamily and a principal receptor for TNF-alpha. It mediates cytokine signaling, activates NF-κB, promotes angiogenesis, and regulates gene expression. The extracellular domain can be released as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.

TNFR1 interacts with BAG4/SODD, TRADD, and TRAF2 proteins, which regulate downstream signaling. Mutations in TNFR1 extracellular domains are associated with autosomal dominant periodic fever syndrome, likely due to impaired receptor clearance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.