Thermo Fisher Scientific TSLP Monoclonal Antibody (eBio28F12 (28F12)), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

Description:
The monoclonal antibody eBio28F12 reacts with and inhibits the bioactivity of mouse TSLP.
Thymic Stromal-Derived Lymphopoietin (TSLP) was first identified in thymic stromal cell culture supernatant as a growth factor for pre-B cell proliferation and differentiation. It is structurally and functionally related to IL-7 and induces dendritic cells to promote Th2 differentiation and regulatory T cell development.

TSLP expression is induced by inflammatory cytokines and TLR ligands, regulated by NF-κB, and detected in epithelial, stromal, and basophil cells. Elevated TSLP levels are linked to asthma and atopic dermatitis, both Th2-mediated conditions. Chronic overexpression may increase allergen sensitivity.

Applications Reported:
The monoclonal antibody eBio28F12 reacts with and inhibits mouse TSLP bioactivity.

Applications Tested:
The ND₅₀ of eBio28F12, determined by neutralization of recombinant mouse TSLP-induced proliferation of the Nag8/7 cell line, is 1–3 ng/mL in the presence of 0.5 ng/mL mTSLP. The neutralization dose varies depending on cell type, assay method, and cytokine concentration.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody, determined by LAL assay.

Aggregation:
<10%, determined by HPLC.

Target Information

TSLP is a 159 amino acid four-helix bundle hematopoietic cytokine that signals through a heterodimeric receptor complex of the TSLP receptor and IL-7Rα. It affects myeloid cells, induces chemokine release from monocytes, enhances dendritic cell maturation, and promotes differentiation of naive CD4⁺ and CD8⁺ T cells into proallergic effectors.

TSLP primes dendritic cells to produce IL-12 following CD40 ligand stimulation, influences regulatory T cell selection, and maintains peripheral CD4⁺ T cell homeostasis. It can induce allergic inflammation via mast cell activation and may play a role in inflammatory arthritis. Expression is found in heart, liver, and prostate tissues.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.