Thermo Fisher Scientific SREBP1 Monoclonal Antibody (2A4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 22 publications 22 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Chicken, Hamster, Human, Mouse, Rat

Published species

Avian, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

2A4

Immunogen

6 His-tag fusion protein of SREBP1 corresponding to amino acids 301-407

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11152118

Product Specific Information

In Western blot, a band can be seen at 125 kDa (precursor) and additional bands may be seen at 60-70 kDa (cleaved).

Suggested positive control: IMR 5 and HeLa cells..

Target Information

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.