Thermo Fisher Scientific GNAS Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: A431, Mouse brain
• Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (91%), Xenopus tropicalis (90%), Dog (100%), Pig (100%), Bovine (99%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Mutations in the GNAS gene result in pseudohypoparathyroidism type 1a and 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseous heteroplasia, polyostotic fibrous dysplasia of bone, and certain pituitary tumors.
This gene exhibits complex imprinted expression patterns, encoding maternally, paternally, and biallelically expressed proteins derived from alternatively spliced transcripts. Each upstream exon resides within a differentially methylated region, typical of imprinted genes. The close proximity (14 kb) of two oppositely expressed promoter regions is unusual.
One alternate 5' exon introduces a frameshift, producing an isoform structurally unrelated to others. An antisense transcript may regulate imprinting in this region. Mutations cause pseudohypoparathyroidism type 1a (PHP1a), which shows atypical autosomal dominant inheritance requiring maternal transmission for full penetrance.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.