Thermo Fisher Scientific HMGB1 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

HMGB1 (High-mobility group box-1) is a nuclear non-histone DNA-binding protein. It can be released from damaged or necrotic cells, acting as a proinflammatory cytokine.
Mouse HMGB1 is a 215 amino acid single-chain polypeptide with three domains:

• Two positively charged DNA-binding domains (HMG box A: aa 9–79, box B: aa 89–162)
• A negatively charged 30 aa C-terminal acidic tail region
  Residues 28–44 and 180–185 contain nuclear localization signals (NLS).
  The B box mediates cytokine activity, while the A box is associated with transcription factor binding. HMGB1 promotes monocyte migration, cytokine secretion, and mediates T cell–dendritic cell interactions.
  It signals through receptors such as RAGE, TLR2, and TLR4. HMGB1 is highly conserved and ubiquitously expressed in nuclei and cytoplasm of most cell types, acting as a key mediator of sterile and infection-associated inflammation.
  Elevated HMGB1 levels are observed in tissue injury, infection, sepsis, arthritis, and ischemia-reperfusion injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.