Thermo Fisher Scientific HLA-DQB1 Polyclonal Antibody

Applications

Product Specifications

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins.
Class II molecules are expressed in antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26–28 kDa and encoded by six exons:

• Exon 1: leader peptide
• Exons 2 and 3: extracellular domains
• Exon 4: transmembrane domain
• Exon 5: cytoplasmic tail

Within the DR molecule, the beta chain contains all polymorphisms specifying peptide binding specificities. Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
DRB1 is expressed at levels five times higher than its paralogs DRB3, DRB4, and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4, and DRB5. There are four related pseudogenes: DRB2, DRB6, DRB7, DRB8, and DRB9.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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