Thermo Fisher Scientific HSP60 Monoclonal Antibody (2E1/53)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-10 µg/mL

-

Immunocytochemistry (ICC/IF)

1:20-1:200

-

ELISA (ELISA)

1-10 µg/mL

-

Immunoprecipitation (IP)

2 µg

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Rabbit

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

2E1/53

Immunogen

Human placental HSP60.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325461

Product Specific Information

MA3-013 detects HSP60 from human, monkey, mouse, and rat samples. This antibody displays no other protein reactivity.

MA3-013 has been successfully used in Western blot, immunoprecipitation, immunohistochemistry, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a single 58 kDa protein representing recombinant human HSP60.

The MA3-013 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds between amino acids 211-288.

Reconstitute with PBS.

Target Information

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.