Thermo Fisher Scientific HSP60 Monoclonal Antibody (2E1/53)
HSP60 단백질을 인식하는 Thermo Fisher Scientific의 단일클론 항체로, Western blot, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. 인간, 마우스, 원숭이, 랫트 시료에서 반응하며, IgM 아이소타입의 비결합 항체입니다. 동결건조 형태로 제공되며 안정적인 저장이 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Concentration | Publications |
|---|---|---|
| Western Blot (WB) | 1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 1:20–1:200 | - |
| ELISA | 1–10 µg/mL | - |
| Immunoprecipitation (IP) | 2 µg | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Rabbit |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2E1/53 |
| Immunogen | Human placental HSP60 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_325461 |
Product Specific Information
MA3-013 detects HSP60 from human, monkey, mouse, and rat samples, showing no cross-reactivity with other proteins.
It has been validated for use in Western blot, immunoprecipitation, immunohistochemistry, immunofluorescence, and ELISA.
By Western blot, it detects a single 58 kDa band corresponding to recombinant human HSP60.
The antigen is human placental HSP60. Epitope mapping indicates binding between amino acids 211–288.
Reconstitute with PBS before use.
Target Information
This gene encodes a mitochondrial chaperonin protein essential for folding and assembly of newly imported proteins.
It may function as a signaling molecule in the innate immune system.
The genomic region between this gene and a related family member acts as a bidirectional promoter.
Several pseudogenes are associated with this locus.
Two transcript variants encoding the same protein have been identified.
Mutations in this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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