Thermo Fisher Scientific GRP94 Monoclonal Antibody (9G10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunocytochemistry (ICC/IF)

1:250

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Bovine, Chicken, Hamster, Human, Mouse, Rat

Published species

Chicken, Hamster, Human, Mouse, Pig, Rat, Xenopus

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9G10

Immunogen

Chick oviduct GRP94. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GRP94,uniProtId:P08110-1,ncbiNodeId:9031,antigenRange:1-795,antigenLength:795,antigenImageFileName:MA3-016_GRP94_P08110-1_Rat.svg,antigenImageFileNamePDP:MA3-016_GRP94_P08110-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2248666

Product Specific Information

MA3-016 detects glucose regulated protein 94 kDa (GRP94) from human, rat, mouse, chick, hamster and bovine tissues. MA3-016 is also expected to react with GRP94 from Xenopus, human, canine, rabbit, non-human primate, canine, Drosophila, and ovine tissues.

MA3-016 has been successfully used in Western blot, Immunohistochemistry (paraffin), immunocytochemistry, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~108 kDa protein representing GRP94 in chick oviduct cytosol.

The MA3-016 immunogen is chick oviduct GRP94.

Target Information

GRP94 is a 803 amino acid protein belonging to the heat shock protein 90 family. It acts as a molecular chaperone that functions in the processing and transport of secreted proteins. GRP94 and its N-terminal fragment stimulates CTL expansion and maturation of human monocyte-derived dendritic cells (MDDC). It plays a central role in innate as well as acquired immunity, maturation and chemotaxis of dendritic cells, Ab production, cross-priming, as a potential marker in breast cancer and is a peptide acceptor in endoplasmic reticulum and an accessory to peptide loading of MHC class I molecules. Expression of GRP94 suppressed A23187-induced apoptosis and stabilized calcium homeostasis. GRP94 is expressed in melanoma or liver metastases of colon carcinoma cells, human gastric carcinoma BGC-823 cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.