Thermo Fisher Scientific ADAR1 Recombinant Rabbit Monoclonal Antibody (7H12)

Applications and Tested Dilution

Product Specifications

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), a process known as A-to-I RNA editing. This modification can affect gene expression and function by altering mRNA translation, pre-mRNA splicing, RNA stability, and RNA structure-dependent activities such as microRNA processing or protein-RNA interactions.

ADAR can edit both viral and cellular RNAs, either at multiple sites (hyper-editing) or specific sites (site-specific editing). Cellular RNA substrates include BLCAP, GRIA2, HTR2C, and GABRA3, where site-specific editing leads to amino acid substitutions that alter protein function.

Viral RNA substrates include HCV, VSV, MV, HDV, and HIV-1. ADAR1 can have proviral or antiviral effects depending on the virus and mechanism—either editing-dependent or editing-independent. For example, it impairs HCV replication via RNA editing, enhances replication of MV, VSV, and HIV-1 through suppression of EIF2AK2/PKR, and promotes HDV replication through A-to-I editing of the amber/W site, enabling synthesis of the large delta antigen (L-HDAg). However, excessive ADAR1 expression can inhibit HDV replication.

(Source: Uniprot)

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.